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Report of a Vegetables Network. Second Meeting, 26–28 June 2007,
Olomouc, Czech Republic
PART I - DRAFT (PDF file 484KB)
Workplan agreed in June 2007
The European Brassica Database
Brassica Genome Gateway
Brassica
Working Group members
Brassica
Meetings & Reports
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Background information
The formation of a Working Group on Brassica was decided in 1989 and the
Group first met in 1991. The Group has developed and updated workplans at successive meetings.
The last meeting (see: Report of the meeting) of the Brassica Working Group was held jointly with
the third meeting of the EU funded project GEN RES 109-112 in Vila Real, Portugal, in February 2002.
The Group also met in a parallel session with the other Vegetables Network Working Groups (see: report [here],
PDF file 850KB), during the Vegetables Network meeting, held in Skierniewice, Poland, in May 2003. As well,
the Group met in a parallel session during the Second meeting of the Vegetables Network
in Olomouc, Czech Republic, on 26–28 June 2007. On this occasion a new
workplan was agreed by the Group members.
See article:
Activities and achievements of the ECPGR Brassica Working Group, L. Maggioni.
ISHS Acta Horticulturae 459:
International Symposium Brassica 97, Xth Crucifer Genetics Workshop
The European Database for Brassica (Bras-EDB)
From the very beginning, the Group recognized the central importance of a database in
the development of its activities, as a management tool to prevent genetic erosion and make
existing germplasm accessible. The initial steps to develop a European database of cultivated
Brassica accessions was taken by the Plant Breeding and Acclimatization Institute (IHAR),
Poznan, Poland and a wild species database was developed by C. Gomez-Campo (Spain) and M.
Gustafsson (Sweden).
The management of the
European Database for Brassica (Bras-EDB) was transferred
in 1991 to the Centre for Genetic Resources, the Netherlands. The database focuses on passport data of
collections of the Genus Brassica maintained in European countries.
The latest version of the Bras-EDB was updated with funding from the GENRES CT99 109-112 project on
Brassica (2000-2003), it now includes 36 collections of 22 countries and contains more than
19 600 accessions. The information is centralized in a format based on the FAO/IPGRI Multi-Crop Passport
Descriptors (MCPDv2, December 2001). The updated Bras-EDB is searchable on-line since mid 2004.
The enormous improvement of the Bras-EDB, with an addition of more
than 6500 accessions allows optimal access of the European Brassica collections to
potential users. This specific crop database is rather unique and the linkage of the evaluation
and characterization results of this GENRES project makes the Bras-EDB even more useful for future research.
See article:
The European Brassica Database, Ietje W. Boukema, Theo J.L. van Hintum.
ISHS Acta Horticulturae 459:
International Symposium Brassica 97, Xth Crucifer Genetics Workshop
Safety-duplication
The objective of safety-duplication of the
accessions is a matter of concern for the Group. CGN, the Netherlands and Horticulture
Research International (HRI), Wellesbourne, UK have maintained
responsibility for International Brassica Base Collections since the
1980s and are reciprocally safety-duplicating the accessions conserved.
The concept of a "black box" arrangement, whereby the genebank of
origin holds the responsibility for the quality of the stored material and
its regeneration when required, is considered the most cost-effective method
of safety-duplication.
The following is a list of genebanks offering to host safety-duplicates:
CGN,
The Netherlands
The
Czech Genebank, RICP, Prague-Ruzyne
HRI,
Wellesbourne, UK
The
Nordic Gene Bank
The
Spanish Genebank
It is recommended that:
Samples
sent for safety-duplication should contain a minimum of 300 seeds
The
bilateral arrangement should be made with institutes offering optimum
long-term storage conditions (-20ºC, etc.)
The safety-duplication of Brassica collections in Europe has been highly
improved during the GENRES CT99 109-112 project period (2000-2003). The majority of the genebanks
have now safety duplicated their Brassica collections in another place.
Regeneration guidelines
Procedures for regeneration are a matter of concern for Brassica curators.
Studies carried out within the Group have shown that unexpected changes of gene frequencies occur
following the normal regeneration practices. Although there is still limited scientific background to
establish objective criteria to adopt for the regeneration of each Brassica species, the Working
Group agreed that the following minimum guidelines should be followed for the regeneration of
Brassica accessions:
Use
not less than 50-60 plants per accession
Undertake
either controlled pollination inside isolation cages or tunnels (making use of pollinator insects),
or allow for outdoor open pollination, with respect of a minimum geographical distance
Give
priority to regeneration of unique accessions of national origin. The Bras-EDB should be checked
to verify the uniqueness or the level of duplication of accessions in the European collections
Link with EU project
The GENRES CT99 109-112 project “Brassica Collections for Broadening Agricultural Use,
including Characterizing and Utilising genetic variation in Brassica carinata for its
exploitation as an oilseed crop” was carried out from 2000 to 2003, under the EC regulation 1467/94.
The major aims were to conserve, document, characterize, evaluate and rationalize European collections
of B. oleracea, B. rapa, B. napus and B. carinata accessions.
The project was complementary to the activities of the ECPGR Working Group on Brassica.
A core collection was developed for each group of species and all accessions included in the four
core collections have been tagged in the Bras-EDB. The project has given much emphasis on regeneration,
characterization and evaluation of germplasm included in the four core collections. The cores and
other parts of the collections were characterized for the minimum sets of descriptors.
The involvement of Genebank partners, as well as private breeding companies, universities and institutes
from different European countries represents a guarantee for the future use of Brassica
germplasm in the development of new varieties for European agriculture.
In situ conservation of wild Brassica
The implementation of a strategy for in situ
conservation of wild species of the Brassica oleracea cytodeme
has been suggested by the Working Group as a complementary way of preserving
the diversity of these Mediterranean relatives of cultivated Brassica.
Priority was assigned to the Sicilian center of diversity, where the level
of variability is very high and the populations of B. macrocarpa, B.
rupestris and B. villosa are often threatened by human activities
like quarrying, fires, overgrazing, construction and competition by alien
species.
The Working Group feels that protection
in genetic reserves should be driven by the local authorities (state,
region, etc.). The role of the Working Group is seen as a contribution to
highlighting the usefulness of the wild germplasm for breeding purposes.
During the Vegetables Network meeting in Poland, the intention to have a project on
resistance to different pests and diseases in wild Brassica, which could be used in
breeding, was discussed. A survey of collections through a questionnaire has been sent to the
collection holders, in order to have an overview on the availability of wild Brassica
accessions in Europe and to assess whether collection missions will be necessary. An analysis of the
results is expected after 30 April 2004.
For more information or comments:
Please contact the Chair of the Working Group: Mr Ferdinando Branca
[click here]
and Vice-Chair: Ms Noortje Bas [click here]
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