ECPGR Homepage



Brassica Working Group

Vegetables Network

Report of a Vegetables Network.
Second Meeting,
26–28 June 2007,
Olomouc,
Czech Republic
[PDF file 1.8MB]



Workplan agreed in June 2007

Project document for Phase VIII







The European Brassica Database

Brassica Genome Gateway


Brassica Working Group members

 


  Background information   Regeneration guidelines
  The European Database for Brassica   Link with EU project
  Safety-duplication   In situ conservation of wild Brassica
 

Recent events

The Brassica Working Group held its 4th meeting in Linguaglossa, Catania, Italy, on 2-4 March 2010.
Presentations given during the meeting

Background information

The formation of a Working Group on Brassica was decided in 1989 and the Group first met in 1991. The Group has developed and updated workplans at successive meetings. The last meeting (see: Report of the meeting) of the Brassica Working Group was held jointly with the third meeting of the EU funded project GEN RES 109-112 in Vila Real, Portugal, in February 2002.
The Group also met in a parallel session with the other Vegetables Network Working Groups (see: report [here], PDF file 850KB), during the Vegetables Network meeting, held in Skierniewice, Poland, in May 2003. As well, the Group met in a parallel session during the Second meeting of the Vegetables Network in Olomouc, Czech Republic, on 26–28 June 2007. On this occasion a new workplan was agreed by the Group members.

See article: Activities and achievements of the ECPGR Brassica Working Group, L. Maggioni. ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop

The European Database for Brassica (Bras-EDB)

From the very beginning, the Group recognized the central importance of a database in the development of its activities, as a management tool to prevent genetic erosion and make existing germplasm accessible. The initial steps to develop a European database of cultivated Brassica accessions was taken by the Plant Breeding and Acclimatization Institute (IHAR), Poznan, Poland and a wild species database was developed by C. Gomez-Campo (Spain) and M. Gustafsson (Sweden).

The management of the European Database for Brassica (Bras-EDB) was transferred in 1991 to the Centre for Genetic Resources, the Netherlands. The database focuses on passport data of collections of the Genus Brassica maintained in European countries.
The latest version of the Bras-EDB was updated with funding from the GENRES CT99 109-112 project on Brassica (2000-2003), it now includes 36 collections of 22 countries and contains more than 19 600 accessions. The information is centralized in a format based on the FAO/IPGRI Multi-Crop Passport Descriptors (MCPDv2, December 2001). The updated Bras-EDB is searchable on-line since mid 2004.

The enormous improvement of the Bras-EDB, with an addition of more than 6500 accessions allows optimal access of the European Brassica collections to potential users. This specific crop database is rather unique and the linkage of the evaluation and characterization results of this GENRES project makes the Bras-EDB even more useful for future research.

See article: The European Brassica Database, Ietje W. Boukema, Theo J.L. van Hintum. ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop

Safety-duplication

The objective of safety-duplication of the accessions is a matter of concern for the Group. CGN, the Netherlands and Horticulture Research International (HRI), Wellesbourne, UK have maintained responsibility for International Brassica Base Collections since the 1980s and are reciprocally safety-duplicating the accessions conserved. The concept of a "black box" arrangement, whereby the genebank of origin holds the responsibility for the quality of the stored material and its regeneration when required, is considered the most cost-effective method of safety-duplication.
The following is a list of genebanks offering to host safety-duplicates:

CGN, The Netherlands
The Czech Genebank, RICP, Prague-Ruzyne
HRI, Wellesbourne, UK
NordGen (formerly the Nordic Gene Bank, NGB), Sweden
The Spanish Genebank

It is recommended that:

 Samples sent for safety-duplication should contain a minimum of 300 seeds
 The bilateral arrangement should be made with institutes offering optimum long-term storage conditions (-20ºC, etc.)

The safety-duplication of Brassica collections in Europe has been highly improved during the GENRES CT99 109-112 project period (2000-2003). The majority of the genebanks have now safety duplicated their Brassica collections in another place.

Regeneration guidelines

Procedures for regeneration are a matter of concern for Brassica curators. Studies carried out within the Group have shown that unexpected changes of gene frequencies occur following the normal regeneration practices. Although there is still limited scientific background to establish objective criteria to adopt for the regeneration of each Brassica species, the Working Group agreed that the following minimum guidelines should be followed for the regeneration of Brassica accessions:

Use not less than 50-60 plants per accession
Undertake either controlled pollination inside isolation cages or tunnels (making use of pollinator insects), or allow for outdoor open pollination, with respect of a minimum geographical distance
Give priority to regeneration of unique accessions of national origin. The Bras-EDB should be checked to verify the uniqueness or the level of duplication of accessions in the European collections

Link with EU project

The GENRES CT99 109-112 project “Brassica Collections for Broadening Agricultural Use, including Characterizing and Utilising genetic variation in Brassica carinata for its exploitation as an oilseed crop” was carried out from 2000 to 2003, under the EC regulation 1467/94. The major aims were to conserve, document, characterize, evaluate and rationalize European collections of B. oleracea, B. rapa, B. napus and B. carinata accessions.
The project was complementary to the activities of the ECPGR Working Group on Brassica.

A core collection was developed for each group of species and all accessions included in the four core collections have been tagged in the Bras-EDB. The project has given much emphasis on regeneration, characterization and evaluation of germplasm included in the four core collections. The cores and other parts of the collections were characterized for the minimum sets of descriptors.

The involvement of Genebank partners, as well as private breeding companies, universities and institutes from different European countries represents a guarantee for the future use of Brassica germplasm in the development of new varieties for European agriculture.

In situ conservation of wild Brassica

The implementation of a strategy for in situ conservation of wild species of the Brassica oleracea cytodeme has been suggested by the Working Group as a complementary way of preserving the diversity of these Mediterranean relatives of cultivated Brassica. Priority was assigned to the Sicilian center of diversity, where the level of variability is very high and the populations of B. macrocarpa, B. rupestris and B. villosa are often threatened by human activities like quarrying, fires, overgrazing, construction and competition by alien species.

The Working Group feels that protection in genetic reserves should be driven by the local authorities (state, region, etc.). The role of the Working Group is seen as a contribution to highlighting the usefulness of the wild germplasm for breeding purposes.
During the Vegetables Network meeting in Poland, the intention to have a project on resistance to different pests and diseases in wild Brassica, which could be used in breeding, was discussed. A survey of collections through a questionnaire has been sent to the collection holders, in order to have an overview on the availability of wild Brassica accessions in Europe and to assess whether collection missions will be necessary. An analysis of the results is expected after 30 April 2004.



For more information or comments:
Please contact the Chair of the Working Group: Mr Ferdinando Branca [click here] and
Vice-Chair: Ms Noortje Bas [click here]